鸡16号染色体SNP单倍型和拷贝数变异对血清IgG含量的影响

doi:10.11843/j.issn.0366-6964.2013.09.004

Abstract

Abstract:

This study was performed to examine the haplotypes and copy number variation (CNV) of genome in chromosome 16 of Beijing-You chicken and their association with IgG level. A total of 995 chickens were genotyped using 60 K Beadchip and IgG level were measured at 80 d in serum. PennCNV, Haploview 4.1 and PHASE 2.0 were used to analyze CNV, linkage disequilibrium (LD) and haplotypes. The results represent two CNVs and two Blocks. In Block1, three haplotypes and six diplotypes were observed where IgG level of H1H2 was 1.45 times as H3H3 (P<0.05). In Block2, nine haplotypes and nine normal diplotypes were analyzed, but no significant difference were identified (P>0.05). Detection of copy number variations at GGA16（201 882-299 166 bp）was 1.25 times as that of the duplication of this region (P<0.05). Eight SNPs markers were chosen for analysis. The SNP markers Gga_rs16057310, Gga_rs15788237 and Gga_rs15788101 were significantly differed in IgG level among genotypes (P<0.05). In all, Block1, CNV2 (201 882-299 166 bp) and SNPs Gga_rs16057310, Gga_rs15788101, Gga_rs15788237 may be influenced on IgG level. The genes in these regions such as CD1b, TRIM27,ZNF692 and MHC could be related with chicken immunity function.

CLC Number:

S831.2

ZHOU Wei, LIU Ran-ran, ZHENG Mai-qing, WEN Jie, ZHAO Gui-ping,CHEN Guo-hong, CHANG Guo-bin, ZHANG Lei, SUN Yan-fa, LI Peng, SUN Yan. Haplotypes and Copy Number Variation of Genome in Chromosome 16 and Their Association with IgG Level in Chickens[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2013, 44(9): 1355-1362.

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Haplotypes and Copy Number Variation of Genome in Chromosome 16 and Their Association with IgG Level in Chickens

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